Articles tagged ”Biosimilars”

Biosimilar Comparability Assesment using FabRICATOR

March 31, 2017 | References |

Biosimilars are gaining in popularity as patents of innovator drugs are expiring. The analytical strategies to characterize and assess the similarity between the innovator product and the biosimilar often involve liquid chromatography and mass spectrometry (LC/MS). In a recent paper from the Freie Universität Berlin, Montacir et al. studied Rituximab and follow-on molecules using FabRICATOR digestion, reduction, and middle-down LC/MS. Using this approach the researchers found differences in the level of c-terminal lysine clipping of the Fc/2 fragment, a sequence error in the Fd fragment (as previously reported by Beck et al 2014), and a pyro-glutamic acid formation in the light chain.

The comparability assessment of biosimilars and innovator drugs using middle-down LC/MS with FabRICATOR digestion have also been published by the FDA a couple of years ago (Wang et al. 2013). Wang et al. argues that the FabRICATOR middle-down approach is very suitable for rapid fingerprinting of complex molecules, due to the high robustness and specificity of the enzyme.

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Montacir, O. et al., 2017. Comparability study of Rituximab originator and follow-on biopharmaceutical. Journal of Pharmaceutical and Biomedical Analysis. E-published ahead of print.

Wang, B. et al., 2013. Structural comparison of two anti-CD20 monoclonal antibody drug products using middle-down mass spectrometry. The Analyst, 138(10), pp.3058–3065.